Induction of Apoptosis in Ovarian Carcinoma Cell Line by Gonadotropin-releasing Hormone Agonist.

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概要

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• Gonadotropin releasing hormone (GnRH) agonist suppresses the growth of the cancer cells in vitro. To evaluate the effect of a GnRH agonist (GnRHA) in ovarian carcinomas, we investigated the interactions of GnRHA with the KOC-2s human ovarian cancer cells. The addition of GnRHA (10<SUP>-8</SUP>10<SUP>-6</SUP>M) produced an increase 20-30% in the number of cells (p<0.05). GnRHA (10<SUP>-5</SUP>M) produced a slight, statistically insignificant decrease of <10% in the cell count. No DNA fragmentation was produced by GnRHA (10<SUP>-8</SUP>-10<SUP>-6</SUP>M). However, GnRHA (10<SUP>-5</SUP>M) produced internucleosomal cleavage of DNA into fragments with multiples of 180 to 200 bp. This DNA "ladder" pattern is characteristic of a poptosis. The amount of Fas antigen was reduced by each concentration of GnRHa. The addition of GnRHA (10<SUP>-6</SUP>M and 10<SUP>-5</SUP>M) significantly increased the secretion of TNF-α (p<0.001). The time- and dose-dependent effects of GnRHA might be confined to the KOC-2s cells as demonstrated by growth inhibitions and characteristic internucleosomal DNA fragmentation. However, the effects of GnRHA on secretion of TNF-α and the expression of Fas antigen differed. The present results provide a basis for future studies on the mechanism of apoptotic effect of GnRHA.

• 久留米大学医学部 The Kurume Medical Journal 編集部の論文

著者

• MOTOMURA SATORU

  Department of Obstetrics and Gynecology, Kurume University School of Medicine

関連論文

• Laparoscopic Treatment of Ectopic Pregnancies. Reports of Two Cases.:Reports of Two Cases
• Induction of Apoptosis in Ovarian Carcinoma Cell Line by Gonadotropin-releasing Hormone Agonist.

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