A SIMPLE METHOD OF ISOLATION AND PURIFICATION OF MYC. LEPRAEMURIUM FROM INFECTED TISSUES

スポンサーリンク

概要

• 論文の詳細を見る

• In the previous paper (1), a method of isolation of Myc. lepraemurium from infected tissue by means of trypsin digestion prior to the procedure of Hanks' method (2) was described. It is fact, however, that contamination of the tissue component in the final product obtained can not be avoided even through careful procedure. This method has two additional disadvantages; a small yield of the bacilli and it is a complicated procedure.The present paper reports a new and simple isolation and purification method of Myc. lepraemurium, under sterile conditions, from the subcutaneous lepromata of the rat by using trypsin digestion with sodium desoxycholate treatment. The efficiency of this method was determined by observing Ziehl-Neelsen staining, Ziehl Nile blue staining (3) under a light microscope, and electron micrographes. Some enzymatic activities and the remaining infectivity of the final product as compared to that of the starting material were also observed.

• 久留米大学医学部 The Kurume Medical Journal 編集部の論文

著者

• Nakamura Masahiro

  Division Of Healthcare Science Research Laboratory Nisshin Oil Mills Ltd.

• UENO Y.

  Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University

• OSHIMA FUMIO

  Division of Bacteriology, Department of Microbiology, Kurume University School of Medicine

• MITSUFUJI YUTAKA

  Division of Bacteriology, Department of Microbiology, Kurume University School of Medicine

• HIGUCHI MASAHITO

  Division of Bacteriology, Department of Microbiology, Kurume University School of Medicine

• NAKAMURA MASAHIRO

  Division of Bacteriology, Department of Microbiology, Kurume University School of Medicine

• UENO YOSHIE

  Division of Bacteriology, Department of Microbiology, Kurume University School of Medicine

関連論文

• Effects of Dietary Medium-Chain Triacylglycerols on Serum Lipoproteins and Biochemical Parameters in Healthy Men(Food & Nutrition Sience)
• CELL-MYCOBACTERIA-INFECTION SYSTEM : II. THE FACTORS INFLUENCING THE PHAGOCYTOSIS OF MYCOBACTERIA BY L CELLS, ESPECIALLY THE EFFECTS OF OLD TUBERCULIN TREATMENT TO THE CELLS
• Illumination Increases the Affinity of Phosphoenolpyruvate Carboxylase to Bicarbonate in Leaves of a C_4 Plant, Amaranthus hypochondriacus
• Re-oxygenation Improves Hypoxia-induced Pulp Cell Arrest
• A SIMPLE METHOD OF ISOLATION AND PURIFICATION OF MYC. LEPRAEMURIUM FROM INFECTED TISSUES

スポンサーリンク