Purification of cytochrome P 450 induced by phenobarbital from rat liver microsomes and its immunohistochemical study during hepatocarcinogenesis with 2-acetylaminofluorene.

概要

論文の詳細を見る
Purification of cyt P 450-PB which is induced by the phenobarbital was performed from rat liver microsomes and anti-cyt P 450-PB serum was obtained from rabbit. Localization of cyt P 450-PB was studied by immunofluorescent method using the antiserum in rat with normal liver, hyperplastic nodules and hepatocellular carcinoma, to understand the changes of cell propeties during hepatocarcinogenesis.<BR>Cyt P 450-PB in normal rat localized around the central vein. In hyperplastic nodules and hepatocellular carcinoma, specific fluorescent areas of cyt P450-PB was not observed, although a few cells was positive in hepatocellular carcinoma. Pericentral areas of rat liver carrying hyperplastic nodules and hepatocellular carcinoma are less fluorescent than that of normal rat. To comparison with cyt P 450-PB, localization of epoxide hydrase (EH), which is another drug metabolizing enzyme, was also studied by immunohistochemical method. Hyperplastic nodules are positive areas of EH, but only a few cells are positive in hepatocellular carcinoma.<BR>These changes of localization of cyt P 450-PB and EH were characteristic during experimental hepatocarcinogenesis. Whether these changes are based upon phenotype happened during carcinogenesis or results effected by metabolizing of carcinogens is remained to be explored.
社団法人日本肝臓学会の論文