EFECT OF STREPTOMYCIN ON THE CHLOROPHYLL FORMATION IN THE TIMOTHY:I. MORPHOLOGICAL OBSERVATION
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概要
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1) Japanese timothies were cultivated in absorbent cotton containing streptomycin and this disturbed the chlorophyll formation in the plant and the degree of disturbance varied proportionally with the concentration of streptomycin so that white leaves were produced, when the concentration of streptomycin reached a certain degree.2) Petioles of the whitened leaves were undergrown, and roots were also remarkably shortened.3) When streptomycin concentration was below 1mg/cc the germination rate of timothy showed no .significant decline.4) When streptomycin was autoclaved at 120°C under 15 lbs pressure for 30 minutes and deprived of its antibacterial activity, it has lost -the power to disturb chlorophyll formation.5) Microscopical examination of white leaves induced by streptomycin revealed that mesophyll cells in the leaves turned to foamy cells and looked transparent as if cytoplasm had been lost.6) It is considered that streptomycin disturbs chlorophyll formation from the plastid-primordia which are contained in the cytoplasm of the mesophyll cells, and that it also prevents the changing process of chlorophyll into chloroplast.7) The chlorophyll formation was found to be stopped after the contact with streptomycin, while it did not work against already produced chloroplasts.8) Leaf blade grown to a certain grade under the influence of streptomycin remained unchanged with the same appearance in their injury after they were released from the influence and transplanted to water medium, and chlorophyll was not produced in them afterwards.9) The activity same as that of streptomycin was also noticed in kanamycin and viomycin.10) Penicillin, sodium para-amino salicylate, isonicotinic acid hydrazide and pyrazinamide did not exert any remarkable effect upon the chlorophyll formation, within the tested concentration.11) The shrinkage of roots of timothy is not specific to the action of the antibiotics yielded from streptomyces group.The author is indebted to Dr. Hideo Moriyama, the director of this institute, for his many valuable advices and encouragements.
- The Keio Journal of Medicineの論文