Experimental infection of hepatitis A virus in marmoset monkeys; Growth kinetics of the virus and the mechanism of liver cell destruction in vivo.
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概要
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Experimental infection of hepatitis A virus (HAV) in marmosets was carried out for the elucidation of HAV growth kinetics in vivo and pathogenesis of hepatocytes destruction. Marmosets inoculated with HAV revealed the elevation of s-GPT and histological changes similar to human acute virus hepatitis. Hepatitis A antigen (HAAg) was detected in liver homogenate from 7 days post inoculation (p.i.) by radioimmunoassay (RIA) and lasted for 3 weeks. HAV shedding into feces was first detected 9 days p.i., and, though varying the amount, continued to 24 days p.i. Immunoperoxidase staining showed that HAAg existed in hepatocytes of marmosets of 14 days p.i. and that hepatocytes containing HAAg increased the number gradually untill 28 days p.i. close attachments of lymphocytes or Kupffer cells to hepatocytes positive for HAAg were observed according with the elevation of sGPT. Neither HAV nor HAAg was detected in any portion of intestine through the observation period. These findings indicate the liver as primary replication site of HAV and the contribution of cellular immune response to the pathogenesis of hepatitis A.
- 社団法人 日本肝臓学会の論文