Study on separation and primary culture of cirrhotic rat hepatocytes.
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概要
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The separation and primary culture of carbon tetrachrolide-induced cirrhotic rat hepatocytes was studied. The cirrhotic liver was perfused with 500ml of 0.25% collagenase solution for 30 minutes. The viability of the separated hepatocytes was 83.2±6.0% (mean±SD) and 0.84±0.28×10<SUP>7</SUP> viable hepatocytes/g liver weight were obtained (mean±SD). By using culture dishes coated with collagen and fibronectin, the hepatocytes could be cultured for 3 days maintaining 75% cell viability. Hepatocytes cultured for 24 hours maintained the same morphological characteristics as at separation. It was considered that primary culture of cirrhotic rat hepatocytes is useful for study of the biochemical characteristics of the hepatocytes in cirrhosis.
- 社団法人 日本肝臓学会の論文