Detection of hepatitis C virus RNA in hepatocytes in formalin-fixed paraffin embedded liver biopsy specimens, using non-radioactive in situ hybridization.:using non-radioactive <I>in situ</I> hybridization

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概要

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• An <I>in situ</I> hybridization technique using thymine dimer method and synthetic oligodeoxynucleotide probe was applied to detect hepatitis C virus RNA in formalin-fixed paraffin embedded liver biopsy specimens. In order to detect HCV genome, which was presumed to be in low concentration in formalin-fixed paraffin embedded specimens compared with fresh frozen section, excessive amount of short synthetic oligodeoxynucleotide probes and digestion with HCl and proteinase of the specimens were used. In addition, the importance of exclusion of dextran sulfate from hybridization solution and extensive dewaxing were elucidated to reduce background signal. Using this method, existence of HCV RNA and its replicative intermediate in the cytoplasm of hepatocyte in liver biopsy specimen of anti-HCV positive chronic hepatitis patient was observed.

• 社団法人 日本肝臓学会の論文

著者

• 河合 博志

  金沢大学第一内科

関連論文

• Detection of hepatitis C virus RNA in hepatocytes in formalin-fixed paraffin embedded liver biopsy specimens, using non-radioactive in situ hybridization.:using non-radioactive in situ hybridization

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