A fundamental study on objective evaluation of histologic differentiation grades of hepatocellular carcinomas.

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Nuclear DNA contents of hepatocytes and tumor cells were measured by means of microspectrophotometry. Eighty-six tumorous regionS and 44 non-tumorous regions from 27 hepatocellular carcinoma (HCC) cases and 13 control regions were submitted to study. All non-tumorous regions and well differentiated HCCs showed a diploid pattern whereas poorly differentiated HCCs expressed an aneuploid pattern, and moderately differentiated HCCs did not exhibit any constant ploidy pattern. Seven parameters related to nuclear DNA contents were selected, and their diagnostic coefficiencies were investigated. The value for 6 n≤%, a parameter indicating the frequency of polyploid cells, was most effective for determining the histologic grade of malignancy, with a diagnostic accuracy of 87%. The relationship between nuclear DNA content and proliferating cell nuclear antigen labeling index and/or cytologic morphometry did not further contribute to the objective and accurate evaluation of histologic grade of differentiation. From the results obtained, the author concludes that microspectrophotometrical studies of nuclear DNA are not assist in the objective determination of the histologic grade of HCC.
社団法人日本肝臓学会の論文