Experimental studies on tumor immunity in peritoneal metastasis model of hepatocellular cartinomas.

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Ten million FAA-HTCl hepatoma cells injected into the peritoneal cavity are sufficient to kill normal Fischer rats within 6 weeks. Peritoneal exudate cells (PEC) were massively accumulated in peritoneal cavities of sensitized rats as compared to that of control rats. Cell populations of PEC were identified by flow cytometric analysis on 2, 4 and 6 days after tumor inoculation. Percentage of CD8 positive T cells in PEC obtained from sensitized rats was elevated on 4 days after inoculation. Percentage of Macrophage in PEC obtained from sensitized and control rats were elevated 2 days after inoculation. But percentage of NK cells, CD4 positive T cells and B cells remained unchanged. Compared with control rats, killer activity of PEC was significantly increased in the sensitized rats on 4 days after inoculation. Depletion of CD8 positive T cells significantly reduced the killer activity of PEC obtained from sensitized rats, whereas depletion of CD4 positive T cells or macrophage did not affected the killer activity. Furthermore, PEC obtained from sensitized rats showed cytotoxicity against FAA-HTCl cells, whereas they failed to show detectable cytotoxicity against other syngeneic tumor cells or allogeneic hepatoma cells. These data exclusively suggested that tumor-specific cytotoxic T cells obtained from sensitized rats were related to the tumor rejection mechanisms in peritoneal metastasis of hepatoma.
社団法人日本肝臓学会の論文