切断後の肝臓神経の再生に関する免疫組織学的研究

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As liver transplantation advances, growing attentions have been paid to the analysis of nerve regeneration. We have examined regeneration process of the hepatic nerve by means of light and electron microscopic immunohistochemistry of anti-GAP-43 antibody after cutting at 8mm proximal to the portal fissure. In unoperated control rats, GAP-43 immunoreactivity localized in the circumferences of several unmyelinated axons. Immediately after the severance, GAP-43 immuno-reactivity disappeared from the nerve fibers, followed by gradual reapperance 2 days after the operation. GAP-43 immunoreactivity was getting stronger and reached maximal level at 14-21 days after the operation, when all axons showed positive reaction. GAP-43 positive axons decreased in number and recovered to the control level during 42-56 days. Electron microscopic immunohisto-chemistry revealed that the GAP-43 positive axons to correspond to the newly formed ones. These results pointed out that regeneration of severed nerve axons starts at 2 days and completed at 42-56 days after the operation.
社団法人日本肝臓学会の論文