Effect of 2-mercaptoethanol on cyanogen bromide cleavage of proteins.

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概要

• 論文の詳細を見る

• 1. On the cyanogen bromide cleavage of urease in 70% formic acid, addition of 2-mercaptoethanol led to the increase of the cleavage.2. Dithiothreitol and thiodiglycol were not effective for it.3. The cleavage ratio was estimated by gel filtration, N-terminal determina tion and the yield of cleaved peptides.4. A similar effect was observed on phosphorylase b as urease.

• The Keio Journal of Medicineの論文

著者

• SEKITA KIYOSHI

  School of Medicine, Keio University

• MAMIYA GUNJI

  Defence Medical College, Tokorozawa-shi, Saitama-ken

• SEKITA TSUNEJIRO

  School of Medicine, Keio University

関連論文

• Effect of 2-mercaptoethanol on cyanogen bromide cleavage of proteins.

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