Studies on cell-mediated immune functions of patients with gynecologic tumor. Cytotoxic activity to human ovarian cancer cell lines and reactivity to phytohemagglutinin shown by lymphocytes.:CYTOTOXIC ACTIVITY TO HUMAN OVARIAN CANCER CELL LINES AND REACTI
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概要
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Peripheral blood lymphocytes collected from 23 healthy female volunteers, and 76 patients with gynecologic tumors including 33 ovarian cancers and 23 uterine cancers, 99 in total, was used to perform the allogeneic cytotoxicity test (to be referred to as "the test") and to evaluate for the blastogenic response to phytohemagglutinin (to be referred to as "the response"). The test was performed according to the method by Takasugi and Klein. Target cells used were two cell lines derived from human ovarian cancer, namely, HOC-21 from serous cystadenocarcinoma and YS-K from yolk sac tumor. The response was assayed on lymphocytes separated from the peripheral blood and on a very small amount of heparinized whole blood.In the test, the higher the concentration of lymphocyte, the more cytotoxically it functioned. The cytotoxicity of lymphocytes from the patients with ovarian tumors, particularly ovarian cancers, was found to be higher than that from the patients with uterine tumors. Also, high cytotoxic activities were seen in the patients with dermoid cysts of ovary and choriocarcinomas. However, it was noted that the cytotoxicity was not always high in the patients whose histologic type was the same as that of the target cell. Cytotoxicity showed a slight decrease along with a progress of disease, but it was rather higher in recurrent cases.By whole blood assay, the response was lower in the patients with malignant tumors than in the patients with benign tumors, and it was significantly low in advanced and recurrent cases. However, it was as normal as in the healthy subjects by lymphocyte assay.
- The Keio Journal of Medicineの論文