Studies on heterogeneity of cultured cell line (KU-2) derived from human renal cell carcinoma: Morphology, karyotypes and growth kinetics based on analysis of 13 clones.

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The heterogeneity of cell line KU-2 derived from human renal cell carcinoma was studied utilizing single cell cloning method. Thirteen sublines named N-1-N-13 were yielded.Observed morphologically as time lapsed, N-13 was different from other sublines in that it was a group of cells, spindle in shape maintained throughout, with a lower nuclear to cytoplasmic ratio and did not tend to pile up, with lower saturation density and long generation time.Transmission electron microscopy showed different characteristics of each subline in the degree of development of cell surface microvilli and the amount of glycogen granules and lipid droplets in cytoplasm.Karyotype analysis of the sublines showed 6 different modal chromosome numbers, but all of the sublines had common marker chromosomes, while clone N-5 had a proper giant metacentric marker chromosome.Generation time determined by the fraction of labeled mitosis method ranged from 18.7 hr (N-10) to 25.6 hr (N-3), so did growth fraction from 0.83 to 1.00.Cloning of KU-2 cell line revealed its heterogeneity detecting sublines with different biological properties. Subsequent separation of individual sublines with different cell kinetics from the heterogenous cell populations would provide clues to clarification of the role of cell kinetics in relation to other biological phenotypes of cancer cells.
The Keio Journal of Medicineの論文