Immunocytochemical Studies on the Distribution of Various Cytoskeletal Proteins in the Pheripheral Vestibular System
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The immunocytochemical distribution of vari-ous cytoskeletal proteins neurofilament (NF) 68 kD, 160kD, 200kD, cytokeratin (CK) 7, 8, 13, 19, vimentin, glial fibrillary acidic protein (GFAP), and microtubule-associated protein 2 (MAP2), was investigated in vestibular endorgans and vestibular ganglia of guinea pigs. NF proteins (NF 68kD, 160kD, 200kD) were found in afferent nerve fibers and nerve terminals which may correspond to the nerve chalice. In vestibular ganglia, NF 68kD and l60kD were predominant-ly distributed in larger cells, whereas NF 200 kD was found in almost all ganglion cells, CK 8 and 19 were found in supporting cells, dark cells of vestibular endorgans. No expression of CK 7 and 13 was detected in the vestibular periphery. Vimentin was observed in the hair cells (which were distributed in the central region of the endorgans), supporting cells, most connective tissue cells, and Schwann cells of the vestibular ganglion. Although much GFAP was seen in glial cells, no immunoreactivity for GFAP was found in the vestibular periphery. MAP 2 was distributed in almost all ganglion cell bodies and their proximal axons. These highly differentiated staining patterns indicate that these cytoskeletal proteins can be utilized as specific markers for each cell type. Furthermore, they may play distinct roles in the different cell types as well.<BR>In the present study, to determine the effects of ototoxic drugs, we also examined the expression of ctyoskeletal proteins in strepto-mycin-treated guinea pigs.<BR>Immunoreactiveties for NF and vimentin (found in the hair cells) decreased after treatment, but the other cytoskeletal proteins were not affected. These results suggest that neuronal components are the primary site of damage in streptomycin-treated animals.
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