Changes of Ca2+-activated, phospholipid and diacylglycerol-dependent protein kinase activity during histamine release from rat mast cells.

概要

論文の詳細を見る
Rat mast cells were challenged with compound 48/80 or calcium ionophore A23187 and protein kinase C activity in the cell pellets and amount of histamine release in the supernatant was measured after stopping the reaction. Histamine was significantly released from compound 48/80-activated mast cells at 30 sec after stimulation. In parallel with it, protein kinase C activity in the cell pellets increased at 30 sec and 1 min, and returned to basal value at 3 min after the stimulation. When mast cells were incubated with various concentration of 48/80 for 30 sec, the amount of histamine release and the protein kinase C activity increased dependently on the concentration of 48/80. Significant histamine release from A23187-acti-vated mast cells was found at 1 min after stimulation. Also, protein kinase C activity in the cell pellets showed a tendency to increase at 1 min and returned to basal value at 5 min after stimulation.These findings suggest that transient increase of protein kinase C activity may be involved in mast cell activation process.