Development to Term of Pig Blastocysts Cultured <I>In Vitro</I> for 0-3 Days
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概要
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Viability of pig blastocysts following <I>in vitro</I> culture for 0-3 day(s) was assessed after transfer to recipients. Embryos collected on Day 4, 5 and 6 were cultured in a modified Brinsters medium +10% fetal calf serum for 3, 2 and 1 day(s) respectively. Embryos were transferred to Day 6 recipients. Day 7 noncultured blastocysts were transferred as a control. Nine (90%) of 10 recipients with noncultured embryos, 6 (75%) of 8 recipients with embryos cultured for 1 day, 5 (83%) of 6 recipients with embryos cultured for 2 days, and 2 (29%) of 7 recipients with embryos cultured for 3 days farrowed. Embryo survival was 50%, 34%, 35% and 12%, respectively. Sur-vival of noncultured embryos was higher (P<0.05) than that of embryos cultured for 1-3 day(s). Survival of embryos cultured for 3 days was lower (P<0.01) than that of those cultured for 1 or 2 day(s). These results indicate that the viability of cultured pig embryos following transfer de-creases with increasing length of culture <I>in vitro</I>.
- 日本繁殖生物学会の論文