ウシの過排卵処理における高純度FSH製剤の利用とその効果
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概要
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The embryo production in cattle by superovulation treatment with the highly purified porcine FSH preparation containing reduced LH activity (FSH-R, Denka Pharm.) and with the commercially available crude porcine FSH preparation (FSH-P, Denka Pharm.) were compared. Twenty-four Holstein cows were treated with 36AU of FSH-P or FSH-R and PGF<SUB>2α</SUB> in decreasing method. At 6, 7 or 8 days after estrus and insemination, all cows were slaughtered and their ovaries were observed visually (12, FSH-P group; 12, FSH-R group), and 21 cows were flushed and ova/embryos recovered (11, FSH-P group; 10, FSH-R group).<BR>On the results, the mean number (+SD) of corpora lutea and total ova/embryos were not different between FSH-P group and FSH-R group (14.2±10.6 vs 14.7±7.4, 9.1±8.2 vs 10.7±6.8, respectively), however, the fertilized ratio and normal ratio were different significantly (86.0% vs 94.4%, P<0.05; 72.0% vs 82.2%, P<0.05, respectively). And the percentages of cows with 3 or more corpora lutea and normal embryos were both 100% in FSH-R group and 66.7% (P<0.05) and 45.5% (P<0.01) in FSH-P group. The difference was found in the number of follicles and the percentage of cows with unusual follicles between FSH-R group and FSH-P group (10.3±6.5 vs 5.5±3.6, P<0.05; 33.3% vs 0%, P<0.05, respectively).<BR>These results suggested that FSH-R treatment was effective as compared with FSH-P treatment for embryo production in daily cows.
- 日本繁殖生物学会の論文