The Base Sequence Corresponding to DYZ1 is Detectable in the DNA from Many Animal Species.

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概要

• 論文の詳細を見る

• Primers possessing base sequences complementary to the 3512-3534th base (Y1.1) and 78-100th base (Y1.2) of a specific repeat base sequence (DYZ1) on the long of the human Y chromosome were prepared. Using these primers, 1μg of cellular DNA from humans, cattle, pigs, rats and mice (both sexes) and testis DNA from salmon and herring were amplified by Polymerase Chain Reaction. In the case of human cellular DNA, the appearance of amplified DNA fragments (154 bp) following electrophoresis, and 55C and 65C hybridization, was noted only for male DNA. In the case of bovine, swine, rat and mouse DNA, such amplified DNA fragments were seen in both male and female DNA after 55C and 65C hybridization. The DNA fragments were also detected in salmon and herring DNA after 55C hybridization. These results suggest that the DYZ1 region is present in the DNA from many mammalian speces and some fishes but also on an autosome in various mammals with the exception of humans.

• 日本繁殖生物学会の論文

著者

• Iwaya Makoto

  Laboratory Of Bacteriology National Children's Medical Research Center

• Ogawa Shyoso

  Laboratory of Reproduction Engeneering, Department of Animal Production, Meiji University

• OGAWA Shyoso

  Laboratory for Reproduction Engineering, Department of Animal Production

• Saga Masahiko

  St. Marianna University, Medical School, Department of Obstetrics and Gynecology

• Higashi Yohsuke

  Laboratory of Reproduction Engeneering, Department of Animal Production, Meiji University

関連論文

• Gene Expression in Blastocysts Following Direct Injection of DNA into Testis
• Expression of Exogenous DNA in Mouse Embryos Surviving After In Vitro Selection with G-418
• Vitrification of Transgenic Mouse Embryos: Effects of Cryopreservation on the Structure and Expression of Transgene
• Successful In vitro and In vivo Development of Cryopreserved Mouse Oocytes Fertilized by Cryopreserved Mouse Epididymal Spermatozoa
• Stage-Dependent Viability of Mouse Preimplantation Embryos Vitrified with Sugar-Containing Solutions
• The Base Sequence Corresponding to DYZ1 is Detectable in the DNA from Many Animal Species.

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