Human Aortic Smooth Muscle Cells Containing Angiotensin II Type 1 Receptors.
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概要
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We characterized the angiotensin II (All) receptor in human aortic smooth muscle cells (HASMCs). This receptor binds [<SUP>125</SUP>I]Sar<SUP>1</SUP>, Ile<SUP>8</SUP>-angiotensin II with a high affinity of 0.20±0.04 nM and a low capacity of 5.3±0.4 fmol/mg protein (230±17 sites/cell). Based on the K<SUB>1</SUB> values, the ranking order of [<SUP>125</SUP>I]Sar<SUP>1</SUP>, Ile<SUP>8</SUP>-AII binding inhibition was as follows: Sar<SUP>1</SUP>, Ile<SUP>8</SUP>AII > AII > Dup 753 > All > AI » PD 123319. The addition of AII to HASMCs induced a rapid, transient increase in intracellular free Ca<SUP>2+</SUP> concentration followed by a lower, sustained phase. When extracellular Ca<SUP>2+</SUP> was removed by adding 3 mM EGTA, this initial transient increase was not changed, but the sustained phase was abolished. These results revealed AII receptors in HASMCs to be of the type 1 receptor subtype, which induce Ca<SUP>2+</SUP> mobilization mainly from intracellular Ca<SUP>2+</SUP> stores.
- 公益社団法人 日本薬理学会の論文