Fluorescent Probe-Labeled Lipid Microsphere Uptake by Human Endothelial Cells: A Flow Cytometric Study.

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概要

• 論文の詳細を見る

• Lipid microspheres have been used as carriers of drugs such as prostaglandin E<SUB>1</SUB> (lipo-PGE<SUB>1</SUB>) and corticosteroid (liposteroid). Lipo-PGE<SUB>1</SUB> is used for the treatment of chronic arterial occlusive diseases because its activity is far greater than that of free PGE<SUB>1</SUB> in vivo. To verify the fact that the drug carriers, lipid microspheres, are preferentially taken up by endothelial cells, we labeled lipid microspheres with a fluorescent probe, DiI (DiI-LM), and observed them in some in vitro models. Stoichiometric fluorescence was obtainable, and the fluorescence was stable between pH 3.3 and pH 8.9. Human umbilical vein endothelial cells and cells of a human endothelial cell line, ECV304, showed increased uptake of DiI-LM, 81% and 61%, respectively. In contrast, uptakes were less than 7% in human skin fibroblasts, 3T3 cells, and human neutrophils. Prominent perinuclear fluorescence was also observed in endothelial cells by fluorescence microscopy. DiI-LM and flow cytometric analysis will be useful for studies to elucidate the precise mechanism of the selective accumulation of lipid microspheres by cells in blood vessel walls.

• 公益社団法人 日本薬理学会の論文

著者

• MATSUKI Yasunori

  Internal Medicine I, National Defense Medical College

• Hidaka Toshihiko

  Internal Medicine I National Detense Medical College

• Suzuki Kimihiro

  Internal Medicine I

• Nakamura Haruo

  Internal Medicine I National Defense Medical College

• Kawakami Makoto

  Internal Medicine I National Defense Medical College

• Kawaguchi Yasushi

  Internal Medicine I, National Defense Medical College

• Takahashi Kimiko

  Biochemistry II, National Defense Medical College

• Sekiyama Yasushi

  Technology Service Center, Green Cross Corporation

• Mizukami Yuichi

  Technology Service Center, Green Cross Corporation

• Kawagoe Mitsuhiro

  Internal Medicine I, National Defense Medical College

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