BUTANOL EXTRACTS FROM MYELIN FRAGMENTS-II. SOME PROPERTIES OF 5-HYDROXYTRYPTAMINE BINDING
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概要
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The myelin fragments of rat brain stem were treated with butanol-water mixtures, and the butanol extracts (total extract=TE) were incubated with 5×10<SUP>-7</SUP> M of C<SUP>14</SUP>•5-HT. After incubation, protein, lipid phosphorus and radioactivity were analyzed by Sephadex LH<SUB>20</SUB> column chromatography. Two peaks of components eluted in chloroform-methanol 4:1 (peak I and II) showed the binding capacity for C<SUP>14</SUP>•5-HT. The displacement studies with unlabeled 5-HT (5×10<SUP>-4</SUP> M) suggested that peak II was the saturable binding component to 5-HT. On the other hand, butanol extracts from the synaptic membranes of rat brain stem did not show binding for C<SUP>14</SUP>•5-HT. Various compounds were studied to determine their inhibitory effects on the saturable binding of 5-HT to TE. The results indicated that acetylcholine, dopamine and tryptamine inhibited the 5-HT binding but LSD, reserpine, colchicine, vinblastine, 5-hydroxytryptophan (5-HTP) and 5-hydroxy-3-indole acetic acid (5-HIAA) had no effect.
- 社団法人 日本薬理学会の論文
著者
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ISHITANI Ryoichi
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Josai University
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IWAMOTO Takio
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Josai University
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MIYAKAWA Akihisa
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Josai University
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