γ-AMINOBUTYRIC ACID IN THE MURINE BRAIN: MASS FRAGMENTOGRAPHIC ASSAY METHOD AND POST-MORTEM CHANGES
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概要
- 論文の詳細を見る
An improved mass fragmentographic assay method for the determination of γ-aminobutyric acid (GABA) in the brain is described. Applicability of the method was examined in a study of the effect of semicarbazide on GABA levels and in a separate study to confirm post-mortem increase in GABA. The method itself is based on Cattabenis procedure in which GABA is assayed as trimethylsilyl derivative. Three improvements were made: a) application of a more suitable mass spectrometry system for GABA determination; b) use of 6-aminocaproic acid as the internal standard; c) selection of a high intensive ion (m/z 174) for mass fragmentographic analysis. The mass spectrometer used is accurate to as little as 25 pg. GABA levels after semicarbazide treatment decreased 54.4% in rat whole brain and 44.2% in the dosal hippocampus. Rapid post-mortem increases in GABA levels were confirmed by application of the improved assay method; decreases were most clearly observable following microwave irradiation. Post-mortem changes in GABA were observed within 3 min after death, as reported by other researchers.
- 社団法人 日本薬理学会の論文
著者
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Hasegawa Yoshikazu
Department Of Urology Matsunami General Hospital
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Maruyama Yuji
Department Of Neuropsychopharmacology (tsumura) Gunma University School Of Medicine
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ONO Takayasu
Departments of Chemistry, Japan Upjohn Research Laboratories
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Hasegawa Yoshikazu
Japan UpJohn Research Laboratories
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MARUYAMA Yuji
Departments of Pharmacology, Japan Upjohn Research Laboratories
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