Histological Evidence for Dissociation of Lipid Peroxidation and Cell Necrosis in Bromotrichloromethane Hepatotoxicity in the Perfused Rat liver.

スポンサーリンク

概要

• 論文の詳細を見る

• Bromotrichloromethane (CBrCl<SUB>3</SUB>)-induced hepatic lipid peroxidation and cell necrosis were studied histologically and biochemically, using isolated perfused livers from phenobarbital-pretreated rats. Lipid peroxidation was assessed by fuchsin staining of the liver slices and release of thiobarbituric acid reactive substances (TBARS) into the perfusate; necrosis was assessed by trypan blue uptake and lactate dehydrogenase (LDH) leakage. A good correlation was observed between the Schiffpositive reaction and TBARS release under various experimental conditions, supporting the validity of the fuchsin staining method for histological detection of lipid peroxidation. Lobular localization of lipid peroxidation and necrosis was as follows: Under high oxygen supply (95% O<SUB>2</SUB>-saturated buffer), infusion of CBrCl<SUB>3</SUB> caused the Schiffpositive reaction in the pericentral to midzonal hepatocytes, irrespective of the direction of perfusion, but did not produce necrosis. Under low oxygen supply (20% O<SUB>2</SUB>) with retrograde perfusion, dissociation of lipid peroxidation and necrosis was observed, i.e., trypan blue uptake in the periportal zones and Schiff-positive staining in the pericentral hepatocytes. Thus, lipid peroxidation by itself may have a relatively minor role in the development of CBrCl<SUB>3</SUB>-induced acute hepatic cell death.

• 公益社団法人 日本薬理学会の論文

著者

• Masuda Yasusuke

  Division Of Toxicology Niigata College Of Pharmacy

• Yamamori Yasuko

  Division of Toxicology, Niigata College of Pharmacy

関連論文

• The Metabolic and Hemodynamic Effects of Oxethazaine in the Perfused Rar Liver
• Intrahepatic Flow Disturbance : Possibility of a Hidden Cause of Drug Toxicity
• Mechanisms Involved in the Contraction of Intrahepatic Portal Vein Branches by Clomipramine and Oxethazaine in Isolated Perfused Rat Livers
• Ca^ Enhancement of Hemolysis Induced by the Topical Anesthetic Oxethazaine in Vitro
• Examination of Intrahepatic Flow Distribution by Vital Staining With Rhodamine 6G in Isolated Perfused Rat Liver
• Intrahepatic Flow Disturbance as Detected by In Vivo Acridine Orange Staining in Endothelin-1-Treated and Cirrhotic Rats
• K+-Linked Release of Oxidized Glutathione Induced by tert-Butyl Hydroperoxide in Perfused Rat Liver Is Independent of Lipid Peroxidation and Cell Death.
• Histological Evidence for Dissociation of Lipid Peroxidation and Cell Necrosis in Bromotrichloromethane Hepatotoxicity in the Perfused Rat liver.

スポンサーリンク