頭乳嘴腫の艀化鶏卵移植試験とその発生因子について

概要

論文の詳細を見る
Though UIImann and Ishikawa had demonstrated the virus which produced laryngeal papillomas, the virus theory of the development of laryngeal papilloma has not generally been accepted because there has been no further demonstration by others. An attempt was made to demonstrate the causative agent of laryngeal papillomas, by the following experiments.Thirteen samples were obtained from 5 patients with laryngeal papillomas and inoculated on the chorioallantoic membrane of 7_??_8day old chick embryo.A) The fresh specimens (within 2 hours after they were obtained) were inoculated on the chrioallantoic membrane and 7 cases out of 12 developed pocks. A passage method was performed on these and the.pock was found in 1 case out of 3 cases.B) The emulsion of the fresh specimens was prepared grinding in the mortar, filtered through the membrane filter and then inoculated. The pock was found in one case out of 2 cases. A passage method was performed and the pock was found in 1 case.C) The material frozen (-20-C) and kept (7_??_53 days) in the refrigerator was planted. The pock was found in 2 cases out of 3 cases.D) No pock was found on chorioallantoic membrane when the material was boiled in Hanks solution (100-C 60 minutes) prior to the inoculation.Above mentioned results suggest that filtrable agent plays an important role in the development of laryngeal papillomar.
社団法人日本耳鼻咽喉科学会の論文