頭頚部扁平上皮癌細胞に対する自己リンパ球の細胞障害性の研究-1-頭頚部扁部扁平上皮癌細胞の培養

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To investigate the immunological cytotoxicity in human autologous system, we tried to culture squamous cell carcinoma in vitro and successfully established a maxillary cancer cell line. Twenty seven cancer tissues obtained surgically were prepared. Primary growth of tumor cells was successful in sixteen out of the 27 cases. Three out of the 16 cases were successfully transfered to passage, but the number of tumor cells in 13 cases gradually diminished, due to remarkable growth of fibroblasts. After 4 months of the establishment of primary culture, 2 of them were contaminated by fungal organisms. Finally, one case of maxillary cancer, which has still been in culture for nearly 1 year and 9 months (32nd serial subpassage), was established as a cell line of squamous cell carcinoma (KOIMC-011). Ultrastructual observation, chromosomal analysis, growing status and immunological features on cell surface confirmed the successful establishment of the cell line.
社団法人日本耳鼻咽喉科学会の論文