単純ヘルペスウイルスによる実験的内耳病変 : II. 単純ヘルペスウイルスのモルモット内耳接種実験

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Department of Pathology, The Institute of Medical Science, Tokyo University, Tokyo Herpes simplex virus was inoculated through the round window membrane into the perilymphatic spaces of the guinea pig labyrinth. A new method for immunofluorescent antibody staining (formalin fixation, EDTA decalcification and trypsin treatment) was applied to detection of the viral antigens in the labyrinth with good preservation of the delicate structures.Immunofluorescent studies revealed that the viral antigens appeared in various areas of the labrinth. The viral antigens were seen in the perilymphatic, endolymphatic, sensory and ganglion structures of the injected side. Histopathological changes were correspondent with localization of the viral antigens, and characteristic morphological changes of the tectorial membrane were seen in some of the animals.Although there were slight or no pathological changes on the opposite side, the viral antigens were also detected with less fluorescent intensity in the endolymphatic, sensory and ganglion structures.The viral antigens were found in the labyrinth of the opposite side, but we have not yet been able to elucidate its routes and mechanism.Histopathological changes at the late stage of the viral infection were confirmed after longterm observations. The neutralizing antibody titer after intralabyrinthine inoculation was studied compared with that after intraperitoneal inoculation.
社団法人日本耳鼻咽喉科学会の論文

単純ヘルペスウイルスによる実験的内耳病変 : II. 単純ヘルペスウイルスのモルモット内耳接種実験

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