上顎洞原発の人癌細胞の培養

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Malignant tumors of the ear, nose and throat are derived mainly from epithelial cells.The only established cell strain of such neoplasms has been HEp-2. Nobody has ever reported any cell strain derived from the epithelium of the paranasal sinus.We have successfully to cultivated tumor cells originating in paranasal sinuses of one of the 25 patients.A cell line from this patient has passed through i5 transfer generations. The patient was a 48-year-old man with cancer of the left paranasal sinus.The tumor had clinically advanced to the degree of T3N, Mo, and was histologically a differentiated sguamous cell carcinoma with marked cornification. Furthermort the character of the cultured cells was studied for chromosome type, plating efficiency and growth curve.A histological study of the cultured cells was done by a light microscope, a phase contrast microscope and an electron microscope. The results were as follows: 1) Morphologically the cultured cells were polygonal and characterized by nuclei of various sizes, which had 2 or more cells nucleoli, and a high nucleus vs.cytoplasm ratio was noted. 2) The karyotype histogram revealed the presence of 2n and occassionally 4n bodies which caused the wide distribution of the chromosomal number. 3) The plating efficiency with Eagles MEM solution was 42%.The cells were shown to multiply even in specially prepared soft agar suspension. 4) The growth curve showed that a period of 6 days was required for the cells to attain a twentyfold count. These findings strongly suggest that the cultured cells are derived from the malignant tumor of the patient and are of an epithelial origin
社団法人日本耳鼻咽喉科学会の論文

上顎洞原発の人癌細胞の培養

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