扁桃病巣感染症の実験的研究 同種家兎扁桃による実験的研究腎炎について
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概要
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It has been assumed that tonsillogenic nephritis is ascribed to the allergic mechanism resulting from infection with hemolytic streptococci. But a few reports are available of this problem.In the present investigation, rabbits were immunized with the mixture of rabbit tonsils emulsion containing type 4, 6, 12, hemolytic streptococci and Freund's incomplete adjuvant. Injections were given intra-muscular once weekly and continued three times. Analysis was made of the rabbit kidney by means of the hematoxylin-eosin stainning method and the fluorescent labelled antibody technique.The results were as follow;1) All the immunized rabbits showed no proteinuria and haematuria for the period of about 4 to 6 weeks after the last immunization.2) In most of rabbits, antibodies to rabbit tonsil developed, which were detectable by means of the precipitin reaction tecfnique. The height of maximum titers varied from 1:32 to 1:512. Preparation containing type 12, hemolytic streptococci showed the highest activity in producing antibodies to tonsil.3) Most of rabbits immunized with type 4, 12, hemolytic streptococci-tonsil emulsion showed the enlarged glomerulus, the moderate increase in number of nuclei, the hyaline thrombi in glomerular vessels and the swelling in number of endotherial cells.4) A direct immunofluorescent technique was employed for detection of tonsillar antigen in the rabbit kidney.Anti-rabbit tonsil globulin was prepared in the chicken and labelled with fluorescent isothiocyanate.Bright specific fluorescence of antigen depositions appeared in the walls of glomerular vessels.Experimental evidence has been presented that tonsil emulsion added to hemolytic streptococci is capable of producing renal lesion. With respect to the genesis of tonsillogenic nephritis, tonsil infected continuously with hemolytic streptococci could be conceived as the cause of damage of the kidney.
- 社団法人 日本耳鼻咽喉科学会の論文