位相差顕微鏡による海狽コルチ器細胞の死後変化に関する観察

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The author studied postmortal changes of the Cortis organ of guinea pigs, using a phase contrast microscope.Microscopical preparations of the Organ of Corti were made from the animals immediately and 1, 2, 3, 4, 6, 8, 12, 24 hours after death by air embolism. The sensory and supporting cells were respectively sepprated from Cortis organ by means of micro-surgical technique.The results were as follows:1. Until 2-3 hours after death, the external and internal hair cells showed no remarkable changes except for swelling of mitochondria, however, after 3 hours there found a tendency of swelling of cytoplasma, mitochondria and nuclei; the nuclear membrane became brilliant and double contoured. After 12 hours, the cells were deformed and vacuoles were found in the cytoplasma.2. Pillar cells and basilar menbrane were not so markedly changed until 6-12 hours after death. 3. Deiters and Hensens cells showed no remarkable changes within 3-4 hours after death, and thereafter postmortal changes in cytoplasma and nuclei of these cells occurred progressively, but even after 24 hours the cells were not disintegrated.The author concluded that the phase contarst microscope is most available to make morphological observation on true postmortal changes of the Cortis organ without being affected by fiexation and staining.
社団法人日本耳鼻咽喉科学会の論文