Alzheimerβアミロイド蛋白アナログのX線構造解析―β結晶の集合―

概要

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Major proteinacious component in the Alzheimer amyloid plaque is β amyloid protein. The amino-acid sequence of this protein is:<BR>Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-lle-Ala-Thr. Among the analogues studied the residues 11-28 showed well-oriented cross β fiber pattern where wide angle reflections could be indexed by an orthogonal lattice having unit cell dimensions a=9.44Å, b=6.92Å and c=10.76Å. The intensity maxima on the equator as well as on higher layer lines were sampled by a larger period (63Å), indicating that the β crystallites constitute higher assembly. The structure was modeled as a tube where 5 beta crystallites constitute the wall.