アクチノマイシンD-DNA複合体の相互作用
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概要
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The crystal structures of the complexes between d (GAAGCTTC) <SUB>2</SUB> and actinomycin D have been determined in two crystal forms by X-ray diffraction analyses and refined to R-factors of 0.187 (<I>C</I>2 form) and 0.202 (<I>F</I>222 form) at 3.0Å resolution, respectively. In each complex, actinomycin D intercalates between the middle 5- (GC) -3 base pairs from the minor groove site and forms hydrogen bonds and van der Waals contacts with DNA. A molecular modeling study based on the crystal structures and a spectroscopic study of the complex in solution have indicated that actinomycin D may have an affinity for A-DNA than B-DNA in approaching to DNA.