Effects of a BCG Nucleic Acid Fraction on Liver Metastases in a Murine Colon Cancer Model.

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Effect of a BCG nucleic acid fraction (MY-1) on prevention of liver metastases were studied in mice.<BR>Murine colon adenocarcinoma 26 cells (1×10<SUP>4</SUP>) were inoculated into the portal vein of female BALB/c mice. After tumor inoculation 300μg of MY-1 was injected every other day, dividing these mice into two groups depending on injection points, namely, one was injected MY-1 into spleen once and then subcutaneously 6 times (IS group, n=21) and the other subcutaneously 7 times (SC group, n=22). Half of the mice was sacrificed 21 days after inoculation, the number of metastatic nodules was significantly reduced in both IS (32±7, SE) and SC (45±9) as compared to control (111±7). Also the survival time of mice was significantly improved. To investigate the mechanism of the antitumor effects, Winn assay was done by using spleen cells. Nonadherent cells showed strong activity, and it was abolished when the spleen cells were pretreated with anti-L 3 T 4 or anti-Lyt 2 antibody and complement. These results demonstrated perioperative immunoactivation with MY-1 reducing the development of liver metastases in mice and antitumor activity was mainly ascribed to T cell activation in the spleen.
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