Studies on the interleukin-2 in peripheral blood mononuclear cells from patients with inflammatory bowel disease.

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Potential abnormalities in the immunoregulatory mechanism in patients with ulcerative colitis (UC) and Crohn's disease (CD) were studied by analysis of IL-2 production, IL-2 receptor expression and responsiveness of peripheral blood mononuclear cells (PBMC) to exogenous IL-2.<BR>PBMC from 30 patients with UC and 17 patients with CD were stimulated with PHA for 15 h and the culture supernatants were assayed for biological activity of IL-2 using an IL-2 dependent cell line. Furthermore, the rate of PHA-stimulated PBMC possessing IL-2 receptors was measured with anti-IL-2 receptor antibody by flow cytometry and IL-2 responsiveness was evaluated from tritiated thymidine incorporation by stimulated PBMC after culture with additional exogenous recombinant IL-2.<BR>The IL-2 production of PBMC from patients with UC and CD was significantly lower than that in 26 normal controls (UC 4.52±3.58 U/ml, CD 3.06±2.98 U/ml versus 11.0±6.4 U/ml, mean±S. D., respectively). This finding did not vary with disease activity. There were no differences between patients and normal controls in their response to exogenous IL-2, or in the rate of IL-2 receptor-bearing cells.<BR>It was concluded that there is selective disturbance in IL-2 production and/or synthesis of an IL-2 suppressive factor in PBMC from patients with inflammatory bowel disease.
日本大腸肛門病学会の論文