発芽過程における種子のインピ-ダンス測定 (特集細胞・DNA等のマニピュレ-ション)

概要

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The impedances of the dielectric cell containing a seed and deionized water were measured to clarify the changes of a seed in germination process. The impedance of the seed was calculated and a symplasmic resistance, an apoplastmic resistance and a capacitance of plasma membranes were calculated on the basis of a linear equivalent electrical circuit for plants. At the same time, the interior of a seed was directly observed and compared with the impedances.When the absorption of water began, the relative dielectric constant of the tissue increased in the requency range of 5kHz to 700kHz and the conductivity of the tissue increased in the frequency range of 40kHz to 10MHz. The apoplastmic resistance decreased and the symplasmic resistance increased. After those changes the relative dielectric constant increased and the conductivity increased abruptly in the fequency range of 1MHz to 10MHz. The apoplastmic resistance increased and the symplasmic resistance decreased down to approximately zero. The capacitance of plasma membranes increased with increasing time in germination process.
社団法人電気学会の論文