Effects of Serum Amyloid A and Lysophosphatidylcholine on Intracellular Calcium Concentration in Human Coronary Artery Smooth Muscle Cells
スポンサーリンク
概要
- 論文の詳細を見る
Serum amyloid A (SAA), an acute-phase protein, and lysophosphatidylcholine (LPC), an oxidized LDL component, contribute to the physiological processes of atherosclerosis and cardiovascular disease. However, the effects of SAA/LPC on human coronary artery smooth muscle cells (hCASMCs) have not been fully investigated. Therefore, we examined the effects of SAA/LPC on Ca2+/Mg2+ mobilization and its underlying mechanisms in hCASMCs. Intracellular Ca2+/Mg2+ concentration ([Ca2+]i / [Mg2+]i) was measured with fura-2 AM/mag-fura-2 AM. Conventional RT-PCR analysis was also performed. Both SAA and LPC increased [Ca2+]i by Ca2+ entry. The SAA-induced Ca2+ entry was inhibited by Gd3+, SKF96365, and 2-aminoethoxydiphenyl borate (2-APB), a nonselective transient receptor potential (TRP) channel blocker, but not nifedipine. The LPC-induced Ca2+ entry was blocked by Gd3+, but not nifedipine, SKF96365 and 2-APB. U-73122 and PTX prevented the activation of SAA-, but not LPC-induced Ca2+ influx. LPC, but not SAA, increased [Mg2+]i as well as [Ca2+]i. The RT-PCR analysis revealed the expression of TRPC1/4, TRPV1/2/4, and TRPM7/8 mRNA. These results suggest that SAA/LPC activate Ca2+ influx in hCASMCs; SAA activates it via PTX-sensitive G-protein, PLC and TRPC pathways, while LPC activates it independently of these pathways, where TRPM7 may be partly involved. Thus, TRP protein appears to be a target molecule of Ca2+ signaling in hCASMCs elicited by SAA/LPC, which may play roles in coronary muscle dysfunction under pathophysiological and inflammatory conditions such as atherosclerosis.
著者
-
Iida Haruko
Department of ischemic circulatory physiology, the University of Tokyo
-
Kikuchi Hironobu
Department of Cardiovascular Medicine, The University of Tokyo
関連論文
- OE-297 Nitric Oxide Production Enhanced by Amiodarone and N-desethylamiodarone in Human Endothelial Cells(Cardiovascular pharmacology, basic/clinical-1 (H) OE50,Oral Presentation (English),The 70th Anniversary Annual Scientific Meeting of the Japanese Cir
- Molecular Characterization of ATP-sensitive K^+ Channels in Human Coronary Arterial Smooth Muscle Cells (Excitation-contraction Coupling/Ion Channel 2 (A), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Azelnidipine Inhibits the Induction of Inducible Nitric Oxide Synthase in Vascular Smooth Muscle Cells(Cardiovascular Pharmacology, Basic/Clinical 2 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-022 Molecular and Physiological Characteristics of Transient Voltage-dependent K^+ Currents (Kv) in Human Pulmonary Arterial Smooth Muscle Cells(Excitation-Contraction Coupling/Ion Channel (A) : OJ3)(Oral Presentation (Japanese))
- Background Nonselective Cation Currents in Rabbit Coronary Arterial Smooth Muscle Cells
- Inhibitory Effects of Ursodeoxycholic Acid (UDCA) on the Induction of Nitric Oxide Synthase in Vascular Smooth Muscle Cells (VSMCs)
- Non-selective cation currents regulate membrane potential of coronary arterial smooth muscle cells : Increase by lysophosphatidylcholine
- OE-271 Sex Differences of Left Atrial Remodeling in Spontaneously Hypertensive Rats (SHR)(Arrhythmia, basic-2, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-188 Cardiac Rehabilitation Improves Plasma Pentraxin3 Level in Patients with Cardiovascular Disease(OJ32,Exercise Test/Cardiac Rehabilitation (H),Oral Presentation (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- OJ-187 Effects of 6-Week KAATSU Walking Training on Limb Venous Compliance in Elderly Subjects(OJ32,Exercise Test/Cardiac Rehabilitation (H),Oral Presentation (Japanese),The 73rd Annual Scientific Meeting of The Japanese Circulation Society)
- PJ-502 Effects of KAATSU training on fibrinolytic activity in healthy subjects(Exercise test/Cardiac rehabilitation(07)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- OE-292 Function and Role of Voltage-gated Sodium Channel (NaV1.7) Expressed in Aortic Smooth Muscle Cells(Vascular smooth muscle (IHD),Oral Presentation(English),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- OE-335 Effect of Peak and Endurance Exercises on the Number of Circulating Endothelial Progenitor Cells (EPCs)(Exercise test/Cardiac rehabilitation-1, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- PJ-759 Hemodynamic and Autonomic Nervous Responses to the Restriction of Femoral Blood Flow by Kaatsu(Exercise test/Cardiac rehabilitation-6 (IHD) PJ131,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation S
- PJ-479 Reproducibility of Subendocardial Strain Measurements(Echo/Doppler-19, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
- Effects of Serum Amyloid A and Lysophosphatidylcholine on Intracellular Calcium Concentration in Human Coronary Artery Smooth Muscle Cells
- Improvements in Quality of As-Manufactured Fuels for High-Temperature Gas-Cooled Reactors