Role of Calcium and Protein Kinase C in the Activation of T Cells in Takayasu's Arteritis.

概要

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The roles of protein kinase C and calcium in the T cells of patients suffering from Takayasu's arteritis (TA) in response to the mitogens phorbolmyristate acetate (PMA) and streptococcal antigens have been studied. In TA there was an increased basal activity of protein kinase C (1.074±0.223nmoles/mg protein/min) as compared with that of controls (0.570±12) (p<0.001). There was 75% translocation from the cytosol to membrane fraction in response to PMA. Intracellular calcium levels showed increased basal levels in TA (177.07±12.56nmoles) compared with the controls (112.83±10.6nmoles) (p<0.001) and there was a further rise on stimulation, indicating the T cells were in an activated state. There was a positive correlation between the calcium levels and the activity of protein kinase C (r<=0.71, p<0.05). Unlike the situation in patients with rheumatic fever, T cells in TA showed no stimulation in response to streptococcal antigens. The low level of cAMP (1.12±0.169pmoles/million cells) compared with that of controls (1.4±0.03) further supports the role of PKC-calcium in the T cell activation process. These findings suggest activation of the PKC-calcium pathway in TA.
International Heart Journal刊行会の論文