Studies on the Energy Metabolism of Erythrocytes during Artificial Heart Pumping

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It was said that hemolysis could take place in the artificial heart pumping, so that some changes in the red blood cells would be expected as a cause of hemolysis. These changes were caused by the trauma to the erythrocytes. In order to elucidate the energy metabolism of the red blood cells, 4 calves, which survived for 408, 150, 64, and 41 hours after the total replacement by the artificial hearts, were subjected to the analysis of the erythrocytes adenine nucleotides (adenosine-tri-phosphate; ATP, adenosine-di-phosphate; ADP, and adenosine-mono-phosphate; AMP).Analysis of adenine nucleotides were performed by the enzymatic method. Peripheral blood smear was made and stained with Wrights solution. % crenocytes was obtained by this preparation.It was demonstrated that ATP was decreasing and ADP was increasing toward the end of pumping. ATP: ADP ratio went down. AMP showed fluctuation. Close relationship between ATP level and crenocytes was observed.It was considered that glycolytic activities in the red blood cells during the artificial heart pumping were much influenced by its mechanical and physical trauma, so that their ATP contents went down and the crenocytes increased in number.This led to the intravascular and extravascular hemolysis in the artificial hearts pumping.
International Heart Journal刊行会の論文