白血球性発熱抑制因子その細胞下レベルでの局在

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Subcellular fractions of neutrophilic leucocytes collected from rabbit peritoneal exudates were separated by differential centrifugation. The effects of each fractions on pyrogenicity of bacterial lipopoly saccharide were examined. The fever-suppressing activity was found in two fractions, 7, 000g 10m pellet (neutrophilic B granules fraction) and 14, 000g 10m pellet (neutrophilic A glanules fraction). The fever-suppressing factor in 7, 000g pellet was inactivated by the 60°C 30m heat treatment or freezing-and-thawing. The fever-suppressing factor in 14, 000g pellet was inactivated by the 60°C 30m heat treatment, but they were activated with freezing-and-thawing or sonication. Then A granules were sonicated and centrifuged 78, 000g 120m. The fever-suppressing activity was found in 78, 000g pellet of A glanules. Supernatant was fractionated with ammonium sulfate. The activity was appeared in 80% saturated supernatant. Paper-electrophoretical data showed that active substance might be cationic or basic polypeptide. The physiological significancy of this fever-suppressing factors are discussed.
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