Effects of Insulin-like Growth Factor-I and Growth Hormone on Adipose Conversion of Bovine Preadpocytes

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Adipogenic effects of insulin-like growth factor-I (IGF-I) and growth hormone (GH) in combination with insulin were assessed with secondary culture of stromal-vascular cells from bovine intramuscular adipose tissues. The confluent cells were differentiated by treatment with dexamethasone and 1-methyl-3-isobutylxanthine in serum-free Dulbeccos modified Eagles medium/Hams F-12 (1:1;v/v) supplemented with insulin at physiological (1.7nM) and supraphysiological (1.7μM) concentration. Physiological level of insulin was not able to support the differentiation and supraphysiological level of insulin was adequate for adipose conversion. Physiological concentration of IGF-I (10nM), however, induced glycerol-3-phosphate dehydrogenase (GPDH) activity, which is adipose marker, as high as that observed in supraphysiological insulin alone. Whereas, growth hormone (1nM) inhibited the induction of GPDH activity but did not alter the GPDH activity significantly in the presence of supraphysiological insulin. These results suggest that IGF-I, but not GH, is one of the physio-logical adipogenic factors for bovine intramuscular adipose tissues and is likely to be replaced by higher concentration of insulin in culture.
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