Cytodegenerative and growth-inhibitory effects on LY-80 cells by methotrexate and 5-fluorouracil in combination with angiotensin II induced hypertension chemotherapy(IHC).

概要

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IHC which is an application of characteristics of tumor microcirculation for drug delivery, makes possible to increase chemotherapeutic effects. To examine a role of selective enhancement of drug delivery in biochemical modulation (BCM), cytodegenerative and growth inhibitory effects of methotrexate (MTX) and 5-fluorouracil (5-FU) on LY-80 hepatoma cells were investigated. A combination of MTX : 2 mg/kg+5-FU : 20 mg/kg of 5-consecutive-days-administration (day 3∼7 after inoculation)showed the highest cytodegenerative effects on intraperitoneal LY-80 cells, comparing with the other MTX/5-FU and 5-FU/leucovolin (LV)combination. Time interval effect was not obviously defined in this system. Growth inhibitory effect of MTX 2 mg/kg+5-FU 20 mg/kg on subcutaneous tumor which 5×10<SUP>5</SUP> of LY-80 were transplanted, was signifficantly enhanced by IHC, comparing with that of non-IHC in both day 7, 9, 11 and 9, 12, 15 administration groups. LV rescue which 5 mg/kg was administered 1 hour after MTX/5-FU, had prevented inhibitory effect on tumor as well as had reduced weight loss of rats. According to this preliminary results, it showed that enhancement of drug delivery had an important role for chemotherapy using BCM.
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