Effect of Xenon Arc Irradiation on Mink Hair Protein Extracted with Thioglycolate
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概要
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The effect of xenon arc irradiation on the extractability and properties of mink hair protein treated with sodium thioglycolate solution was investigated. Over 50% of the intact sapphire mink hair was dissolved when treated with TG 11 (0.1M sodium thioglycolate, pH 11) and TG/G-7 (0.1M sodium thioglycolate, 6M guanidine-HCI, pH 7), but hardly dissolved with TG/U-7 (0.1M sodium thioglycolate, 7M urea, pH 7). The extractability of underfur was higher than that of guard hair with TG 11 or TG/G-7. The extractability with TG 11 or TG/G-7 was remarkably decreased in intact hair than irradiated hair. This implies that the irradiation-induced deterioration of mink hair involves the formation of some additional intermolecular or interpeptide crosslinks other than the original disulfide bridges in native hair. Amino acid analysis, SDS-PAGE and size exclusion chromatography of TG/G-7 extract revealed that the irradiation of mink hair made preferentially microfibril protein insoluble, and leaves matrix protein especially high-glycine-tyrosine component soluble.
著者
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Imai Tetsuo
Tokyo Metropolitan Leather Technology Center
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TERASHIMA Mariko
Tokyo Metropolitan Leather Technology Center
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CHONAN Yasumasa
Tokyo Metropolitan Leather Technology Center
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SHIRAI Kunio
Scleroprotein and Leather Research Institute, Faculty of Agriculture Tokyo University of Agriculture and Technology
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