Live Detection of Bioluminescent Preimplantation Bovine Embryos

概要

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Early screening of preimplantation bovine embryos was attempted by detecting firefly luciferase gene expression as bioluminescence. No detrimental effect was observed in a 3hr-exposure of 2-cell embryos to 500μM luciferin-containing medium on the subsequent embryonic development to the blastocyst stage, whereas a 6-hr exposure significantly reduced the percentageof the embryos that successfully developed to blastocysts (P<0.05). When the firefly luciferase gene driven by the SV 40 early enhancer and promoter was microinjected in the pronucleus of one-cell stage embryos, clear and strong bioluminescence signals were observed in embryos of four-to 16-cell stages within 10min. The presence of the DNA sequences encoding bovine α-lactalbumin secretion signal significantly enhanced the bioluminescence compared with the basic luciferase gene without the signal sequences (P<0.01). These results suggest that the secretion segnal sequences of bovine α-lactalbumin gene may improve the usefulness of the firefly luciferase reporter gene as a selection means of preimplantation embryos carrying transgenes.
社団法人日本畜産学会の論文