Complete Nucleotide Sequence of a Shiba Goat (Capra hircus) Heat-shock Protein 70-3 (Hsp 70-3) Gene

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The nucleotide sequence of a Shiba goat (Capra hircus) heat-shock protein 70-3 (Hsp 70 -3) gene was determined by the dideoxy-nucleotide chain termination method. The Hsp 70-3 gene was composed of an uninterrupted primary sequence of 2, 696 nucleotides. It composed of a 5 noncoding leader sequence of 491 nucleotides, a 3 noncoding region of 294 nucleotides and a continuous open reading frame (ORF) of 1, 911 nucleotides. The ORF encoded 636 amino acids and the predicted molecular mass was 69, 863 daltons. The multiple potentially regulatory sequences i. e. TATA-box, CCAAT box, GC element and heat shock element (HSE), were found in the 5 flanking region of the goat Hsp 70-3 gene. Comparison of the predicted amino acid sequences of the goat Hsp 70-3 gene with the reported sequence a bovine Hsp 70-3 gene (GenBank accession No. L10428) revealed that the goat Hsp 70-3 was highly identical (up to 98.2%) to the bovine Hsp 70-3 protein, and only 6 out of 636 amino acids were different. The other minor difference was that the goat Hsp 70-3 protein had insertion of 5 amino acids (NKRAV) which were not observed in the bovine Hsp 70-3 protein. We concluded that Capra hircus Hsp 70-3 gene was the homologue of Bos taurus Hsp 70-3 gene considered as one member of the goat Hsp 70 gene family.