構造分析を加えた牧草の組成および栄養価研究-7-赤クローバー生草およびサイレージ中の窒素化合物の組成について
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概要
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In order to compare the distribution of nitrogenous compounds in soilage and silage, this experiment was carried out. Materials used here were the red clover, and they were divided into three parts of plant component (fibrous, concentrated and water soluble parts) by means of the method described in the previous report. The results obtained were as follows:(1) There was a large difference in nitrogen distribution between soilage and silage. In soilage, 26.9% of nitrogen was distributed to the fibrous part, 44.4% to the concentrated part and 28.7% to the water soluble part. On the other hand, in silage, 29.4% of nitrogen was distributed to the fibrous part, 14.5% to the concentrated part and 56.1% to the water soluble part. It was further found that 70% of nitrogen of the concentrated part of fresh red clover was degraded to amino acids and ammonia during the ensiling process, and thus degraded part was shifted to the water soluble part of silage.(2) The amino acid composition of proteins of the concentrated part was determined bythe method of MOORE et al. (1958), and it was resulted that there was no difference betweensoilage and silage.(3) Non-protein nitrogen of the water soluble part of red clover soilage consisted of 4.2%of ammonia-nitrogen, 31.3% of amide-nitrogen, 10.4% of nitrate-nitrogen, 18.3% of aminoacid-nitroged and 18.3% of peptide-nitrogen, and that of silage consisted of 25.6% of ammonianitrogen, trace amide-nitrogen, 1.7% of nitrate-nitrogen, 44.5% of amino acid-nitrogen and no peptide-nitrogen.(4) There was no relationship between the free amino acids in the water soluble part and the amino acid composition of the concentrated part of both soilage and silage. Free amino acids which were found in large quantity in the water soluble part of fresh red clover were aspartic acid (3.1% per total non-protein nitrogen), γ-amino butyric acid (2.5%), serine (2.4%), proline (2.2%), alanine (2.1%) and glutamic acid (1.9%), and those of silage were alanine (7.6%), γ-amino butyric acid (5.4%), leucine (4.5%), glycine (4.2%) and valine (3.9%). Alpha-amino butyric acid, β-amino isobutyric acid and citrulline were found in the water soluble part of silage though they were not found in fresh red clover.
- 公益社団法人 日本畜産学会の論文