牛尿中の17-Hydroxycorticosteroidsに関する研究
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概要
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For the purpose of the Characterization of 17-hydroxycorticosteroids in cow urine, a urine sample was collected from a healthy lactating Holstein cow and treated by a modified Cartlandand Kuizenga's method. The extract obtained was used as a sample for paper chromatography.Paper chromatography was carried out with three solvent systems. Steroids were detected by ultraviolet absorption with the aid of a fluorescent detector (2537 Å). The results obtained were as follows.E=extract of cow urine, HC=hydrocortisone, CS=cortisone.Solvent system:A=xylene and absolute methanol;B=toluene, ethyl acetate, absolute methanol, and water;C=toluene which was saturated with propylene glycol, These results clearly indicate that hydrocortisone, a kind of 17-hydroxycorticosteroids, was contained in the urine of the experimental cow in the present study.No further attempt was made to identify the other small absorptive spots (Rf 0.15) which were separated when the toluene-propylene glycol system was used.
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