High Mobility Group Nucleosomal Binding Domain 2 Protein Protects Bladder Epithelial Cells from Klebsiella pneumoniae Invasion
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概要
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Due to the predominance of multiple-antibiotic-resistant Klebsiella pneumoniae strains, the search for new approaches for the prevention of K. pneumoniae infections is now under intensive investigation. The objective of the present study was to investigate the effects of high mobility group nucleosomal binding domain 2 (HMGN2) protein, which acts on the bladder epithelial cells T 24, on the invasion of K. pneumoniae 03183 and explore its possible mechanisms. Pretreatment with HMGN2 significantly reduced K. pneumoniae 03183 uptake by T 24 cells. In T 24 cells, there were no detectable cytotoxic effects of HMGN2 at any concentration between 32 and 256 μg/ml after 2 h incubation. HMGN2 exhibited no appreciable antibacterial activity against K. pneumoniae 03183. Fluorescence microscopy and flow cytometry analysis revealed that HMGN2 blocked K. pneumoniae 03183-induced actin polymerization. K. pneumoniae 03183-induced phosphorylation of extracellular signal-regulated kinase (ERK) and cofilin were prevented by pretreatment with HMGN2. These results indicated that pretreatment with HMGN2 inhibited cofilin phosphorylation and then induced actin disruption which may block ERK phosphorylation. These changes led to inhibition of K. pneumoniae 03183 invasion of T 24 bladder epithelial cells.
- 公益社団法人 日本薬学会の論文
著者
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Gao Xiang
Department Of Chemistry Fudan University
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Liu Na
Department Of Chemistry And Bacteria Detection School Of Public Health Shandong University
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Cao Yue
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sich
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Wu Guixia
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sich
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Fan Bo
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sich
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Zheng Fengjin
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sich
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Liu Xiaokang
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sich
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Huang Ning
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sich
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Huang Ning
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University
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Gao Xiang
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University
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Cao Yue
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University
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Liu Xiaokang
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University
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Liu Na
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University
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Fan Bo
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University
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Wu Guixia
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University
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Zheng Fengjin
Department of Pathophysiology, West China School of Preclinical Sciences and Forensic Medicine, Sichuan University
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