Metabonomics Study of Brain-Specific Human S100B Transgenic Mice by Using High-Performance Liquid Chromatography Coupled with Quadrupole Time of Flight Mass Spectrometry

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概要

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• For the purpose of investigating the effects of S100B on the development of Parkinsions disease (PD), a high-performance liquid chromatography coupled with electrospray ionization time-of-flight mass spectrometry (HPLC/MS-ESI-TOF) metabonomic approach was established to study the mesencephalon profiling of brain-specific human S100B transgenic mice. In order to obtain more full-scale chemical information of metabolites, two kinds of separation mechanism, including reversed-phase (RP) column chromatography and hydrophilic interaction liquid chromatography (HILIC) column, were combined to use. Acquired data were subjected to principal component analysis (PCA) to investigate the effects of S100B protein on mice mesencephalon metabolite profiles. Potential biomarkers were screened by using Mass Hunter Prossional Profiller (MPP) and were identified by the accurate mass. Twelve metabolites in mesencephalon of S100B transgenic mice were identified as potential biomarkers, among which, glutamic acid (Glu) detected by RP/MS in negative ionization mode, gamma-aminobutyric acid (GABA) and tryptophan (Trp) detected by HILIC/MS in positive ionization mode, phenylalanine (Phe) and histidine (His) detected by HILIC/MS in negative ionization mode, related to metabolic pathway of neurotransmitters in mice central nervous system. The analytical technique used in this paper was able to detect biochemical changes in mesencephalon of S100B transgenic mice, which may be helpful to understand the action mechanism of S100B protein in the development of PD.

• 公益社団法人　日本薬学会の論文

著者

• Xu Yan

  Institute Of Metal Research The Chinese Academy Of Sciences

• Liu Jia

  Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical

• Wang Hai

  Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical

• Zhang Lian

  Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical

• Deng Wei

  Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical

• Zhu Hua

  Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical

• Qin Chuan

  Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical

• Liu Jia

  Institute Of Basic Medical Sciences Qilu Hospital Shandong University

• Wang Hai

  Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Human Disease Animal Model, State Administration of Traditional Chinese Medicine

• Xu Yan

  Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Human Disease Animal Model, State Administration of Traditional Chinese Medicine

• Deng Wei

  Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Human Disease Animal Model, State Administration of Traditional Chinese Medicine

• Qin Chuan

  Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Human Disease Animal Model, State Administration of Traditional Chinese Medicine

• Zhang Lian

  Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Peking Union Medical College, Key Laboratory of Human Disease Animal Model, State Administration of Traditional Chinese Medicine

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• Metabonomics Study of Brain-Specific Human S100B Transgenic Mice by Using High-Performance Liquid Chromatography Coupled with Quadrupole Time of Flight Mass Spectrometry
• Aberrant expression of chemokine receptor CCR4 in human gastric cancer contributes to tumor-induced immunosuppression

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