Natural Plant Extract Tubeimoside I Promotes Apoptosis-Mediated Cell Death in Cultured Human Hepatoma (HepG2) Cells

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概要

• 論文の詳細を見る

• Tubeimoside I (TBMS I), an extract from Chinese herbal medicine Bolbostemma paniculatum (MAXIM.) FRANQUET (Cucurbitaceae) has been shown as a potent anti-tumor agent for a variety of human cancers, but yet to be evaluated for hepatoma that is highly prevalent in Eastern Asian countries including China. Here, we examined in vitro the cytotoxic effects of TBMS I on human hepatoma (HepG2) and normal liver (L-02) cell lines. We also investigated TBMS I-induced molecular events related to apoptosis in HepG2 cells. The results show that TBMS I inhibited the proliferation of both HepG2 and L-02 cells in a dose- and time-dependent manner, but HepG2 cells appeared more sensitive to the agent. When exposed to TBMS I for 24, 48 and 72 h, IC50 for HepG2 cells versus L-02 cells were 15.5 vs. 23.1, 11.7 vs. 16.2, 9.2 vs. 13.1 (μM, p<0.01), respectively. TBMS I induced cell shrinkage, nuclear condensation and fragmentation, cell cycle arrest at the G2/M phase, mitochondrial membrane disruption, release of cytochrome c from the mitochondria, activation of caspase 3 and 9, and shifting Bax/Bcl-2 ratio from being anti-apoptotic to pro-apoptotic, all indicative of initiation and progression of apoptosis involving mitochondrial dysfunction. Taken together, these results indicate for the first time that TBMS I potently inhibited growth in HepG2 cells by mediating a cascade of apoptosis signaling pathways. Considering its sensitivity of HepG2 cells, preferential distribution in the liver and natural product origin, TBMS I therefore may have a great potential as a chemotheraputic drug candidate for hepatoma.

著者

• CHEN Jun

  Key Laboratory of Modern Chinese Medicines, Ministry of Education and Department of Pharmacognosy, C

• Wang Yashu

  Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Bioengineering C

• Deng Linhong

  Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Bioengineering C

• Zhong Hongzhe

  Liaoning Provincial Blood Center

• Wang Yajie

  The 324th Hospital of Peoples Liberation Army

• Jiang Xuemei

  Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Bioengineering C

• Jiang Xuemei

  Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Bioengineering College, Chongqing University

• Wang Yajie

  The 324th Hospital of People's Liberation Army

• Chen Jun

  Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Bioengineering College, Chongqing University

• Wang Yashu

  Key Laboratory of Biorheological Science and Technology, Ministry of Education, and Bioengineering College, Chongqing University

関連論文

• Analysis of interaction property of bioactive components in Danggui Buxue Decoction with protein by microdialysis coupled with HPLC-DAD-MS
• Natural Plant Extract Tubeimoside I Promotes Apoptosis-Mediated Cell Death in Cultured Human Hepatoma (HepG2) Cells

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