各種アミノ酸が精子の呼吸ならびに解糖に及ぼす影響 (体外精子の生存ならびに代謝に影響ある卵黄中の有効成分に関する研究-3,4-)

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The authors have reported in previous papers that the substances in egg yolk stimulating respiration of goat spermatozoa were acetone soluble, dialysable and heat stable and that the dialysable portion of egg yolk contained some amino acids and carbohydrate.This experiment was carried out to clarify the effect of various amino acids on the respiration and aerobic glycolysis of goat spermatozoa. The results were as follows.1. Of the seventeen amino acids used in this experiment, glycine and L-phenylalanine greatly increased the oxygen consumption of spermatozoa, but the latter markdly inhibited the motility of spermatozoa. L-Glutamic acid and L-aspartic acid were slightly effective on the oxygen consumption.2. The addition of amino acids, which were found in the dialysable portion of egg yolk, did not increase the oxygen consumption of spermatozoa.3. The oxygen consumption and aerobic glycolysis of spermatozoa were promoted in the presence of glucose. However, the supplementary addition of amino acids, which were found in the dialysable portion of egg yolk, had no influence on the metabolic activity of spermatozoa.4. From the results mentioned above, it was concluded that the substance in egg yolk primarily effective on the metabolism of goat spermatozoa was glucose.
社団法人日本畜産学会の論文