牛乳凝固酵素に関する研究-1-
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概要
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A colorimetric micromethod for estimating little proteolytic action (pepsin action) contained in milk coagulating enzyme preparations is presented in this paper. The principle of the method consists of cansing tyrosine to react, formed by proteolytic action from casein (substrate), with mercuric sulfate and nitrous acid, estimating colorimetrically the reacting colored substance by a photometer and representing the proteolytic action with γ units of tyrosine from 1mg of enzyme preparations. By this method, commercial rennets, rennet powders prepared by salting out method, papain and some pepsin preparations were compared with one another in their proteolytic action.
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